Chem. Ultimately they concluded that the temperature increment found to be an effective force in disturbing these interactions, mainly between polymer-polymer and polymer-solvent molecules for the used system. Radiofrequency is a well-known medical technique with expanded application in electrosurgery for laparoscopic medical procedures and in dermatology, for sebaceous glands ablation in acne treatment [12, 13], and treatment of photoaging skin symptoms, including wrinkles and hyperpigmentation [14]. (1958). These studies were carried out at the Hisko (Gunpo, Korea). Epub 2021 Feb 9. Molecular weight (M v) and hydrodynamic properties can be calculated from there. Thus, it is a common practice to express the frictional coefficient of rigid structures as. 4D imaging reveals mechanisms of clay-carbon protection and Ioan, C.E. ; Tsuchiya, H.M.; Rist, C.E. In a good solvent, which shows a zero or negative heat of mixing with the polymer, the molecule is less-extended, and the intrinsic viscosity is high. Journal of American Chemical Society, 76 (20), 5041-5052. For data requests, please contact the authors. The skin culture medium was purchased from Biopredic International (Saint-Grgoire, France). Prausnitz MR, Langer R. Transdermal drug delivery. Soc., 21, 1, 78-86. These modifications include degradation of hemidesmosomes attached to the dermis, degradation of desmosomes linked to adjacent cells, contraction of intracellular tonofilament, and lamellipodia formation. For a values from 0-0.5 a rigid sphere is predicted in a ideal solvent, from 0.5-0.8 a random coil in good solvent, and from 0.8-2 a rigid or rod like shape is expected (stiff chain). Harding, Stephen E. (1997). Gillespie, T.; Hulme, M. A. Proc. par | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. Human cadaver skin has been confirmed as alive skin tissue through analysis of its morphology and enzymes, and its usefulness as an alternative skin membrane in drug permeation experiments has been verified [20]. ; Yevlampieva N.P. European Journal of Pharmaceutics and Biopharmaceutics, 68, 319-329. These results are in agreement with a previous study which suggested that the microporation using radiofrequency causes disruption of the SC, enabling the percutaneous permeation of the FITC-dextran [21]. However, considering the trauma and unethical practices associated with animal experiments, in vitro studies with human cadaver skin offers an ethical and viable alternative. Mark-Houwink values confirm that for these conditions dextrans behave as rigid spheres with a tendency at compaction as temperature increases. ; van Nostrum, C.F. Solvent effects on polysaccharide conformation. dextran hydrodynamic Structure Properties of Dextran. Biomaterials Research In order to understand the behavior of this biopolymer in water and to support changes at the M-H parameters with temperature, the hydrodynamic parameters are analyzed (hydrodynamic radius, (RH); Simha number, ((a/b)); Perrin parameter (P); Flory parameter (0); and stiffness chain (dln[]/dT)). Biomater Res 24, 22 (2020). The (a/b) value is 2.5 which confirms that dextran in aqueous solution is a biopolymer with a spherical conformation for an Mw range from 8.8 to 200kDa, and with tendency to compaction with increasing temperature (RH decreases). 2009;301(8):60913. Dextran All possible degrees of curling can be displayed by any molecule, but there will be an average configuration which will depend on the solvent. Equivalent Radii and Ratios of Radii from Solution Properties as Indicators of Macromolecular Conformation, Shape, and Flexibility. Each skin pores was captured using a microscope and the obtained images were analyzed using ImageJ software (National Institutes of Health, Bethesda, USA). Experimental and numerical study on the mixed lubrication WebTry out PMC Labs and tell us what you think. Thomas Gutberlet (Lipoteichoic Acid): Statistiques d'influence Springer Editions. where c is the solute concentration (g/cm3), [] is the intrinsic viscosity (cm3/g), and kH represents Huggins constants (cm3/g). (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. TEWL measurements were taken 30min after microporation of the human and pig skin. Polymolecularity and polydispersity in Molecular weight determinations. At 25C both values of a and kM-H are within the range reported by [3, 8, 16]. The equation 4 can be rearranged and resulted in a modified M-H equation as follows: where the value qM-H is the polydispersity correction factor, and it is a statistical function of the molecular weight distribution. Journal of Controlled Release, 119, 5-24. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Physicochemical studies of oligodextran. The explanation for the anomalous values of a and kM-H is because the mixture solute-solvent is highly compatible due a great compaction of biopolymer, from undisturbed state in the absence of interactions, where free energy of mixing less than zero, and emphasize the nature non-linear or hyperbranched of dextran for Mw high to 200kDa. hydrodynamic Carbohydrate Research, 160, 205-214. 2005;22(4):5505. PMC WebTo address this perception, in the current study, FITC-dextran of different MWs (10, 70 and 150 kDa) was used to model the transdermal delivery of low MW biotherapeutics and mAbs with MWs of approximately 150 kDa. Hydrodynamic Properties of Tragacanthin. (1990). WebIn this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. Figure4 shows the histological analysis of micropores in human cadaver skin. De Belder, A.N. Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation, https://doi.org/10.1186/s40824-020-00201-7, http://creativecommons.org/licenses/by/4.0/, http://creativecommons.org/publicdomain/zero/1.0/. Barshtein, G.; Tamir, I.; Yedgar, S. (1998). 2018;1:10915. Biodegradable dextran hydrogels crosslinked by stereocomplex formation for the controlled release of pharmaceutical proteins. B 113, 8020-8025. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. J. Braz. Chen Rong Huei; Wei Yu Chen; Shang Ta Wang; Chu Hsi Hsu; Min Lang Tsai (2009). ; Ghosh, T.K. A sugar cane native dextran as an innovative functional excipient for the development of pharmaceutical tablets. The optical image and histological analysis confirmed that the micropores on the skin were recovered over time. Pharmaceut Sci Tech. The regeneration process of the skin is regulated by growth factors such as keratinocyte growth factor and fibroblast growth factor, which trigger the proliferation and migration of cells [23, 24]. European Polymer Journal, 37, 619-622. On single point determination of intrinsic viscosity. A novel correlation for protein diffusion coefficients based on molecular weight on radius of gyration. The [] is connected to the dimensions of the biopolymer molecule isolated in a certain solvent. Many works are aimed at determining molecular weights [1, 7, 26], hydrodynamic radius, among other parameters for characterizing the physics and chemistry properties of dextran in solution. ; Rani, K.Y. Progress in Measurement, Interpretation and Application to Structure in Dilute Solution. With FITC-dextran 20K, cumulative permeation in the human skin was 2.530.48M, and 3.340.19M in the pig skin, which significantly lower than FITC-dextran 4K. Cumulative amount of FITC-dextran permeated after microporation on the human and pig skin for FITC-dextran M.W.. a M.W. Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. Nanoparticles in magnetic resonance imaging: from simple to dual 113, 81-86. Characterization of high molecular weight dextran produced by Weissella cibaria CMGDEX3. This conjecture found to be correct. Armstrong, J. K.; Wenby, R. B.; Meiselman, H. J.; Fisher, T. C. (2004). "Dextrans in Aqueous Solution. The first term on the left-hand side of equation 2 (i/c) has a linear relation as a function of the concentration (c in g/cm3), where is the intrinsic viscosity [] is given by (c0) [42, 43]. Amersham Biosciences, AA Edition, www.amershambiosciences.com. Accessibility If plotted for all molecular weights, the double logarithm of [] and Mw, the value of a obtained in all cases shows a value smaller than 0.5, which reports that dextran macromolecule has a degree of hyperbranched with increasing molecular weight and increases much for higher Mw (< 200kDa) which making the application of M-H method more difficult. J Pharm Sci. The regeneration of micropores in the human cadaver skin caused by microporation was confirmed using an optical microscope and haematoxylin/eosin (H&E) staining. Degrees of long-chain branching in dextrans. This result could reflect the presence of some aggregated species within the lysozyme sample solution. Quantitative data are presented as the meanstandard deviation, and comparisons were carried out using one-way ANOVA (Systat Software Inc., Chicago, IL, USA). 7 8 Article 2.It can be found from the figure that at the start-up stage of the shaft, the hydrodynamic effect (HE) is weak, so the HP is not enough to support the shaft, which Molecular Weight Distributions of Water-Soluble Polymers by Flow Field-Flow Fractionation. Ed., 9(2), 85-90. The genus Weissella: taxonomy, ecology and biotechnological potential. To overcome these limitations, microporation devices that act as therapeutic vehicles for delivering drug molecules across the skin barriers have been developed. The Dextrans intrinsic viscosity is determined as a function of the molecular weight in water and NaOH 0.5 M see reference [1]. Salunkhe SS, Bhatia NM, Pokharkar VB, Thorat JD, Bhatia MS. Topical delivery of idebenone using nanostructured lipid carriers: evaluations of sun-protection and anti-oxidant effects. Coviello, T.; Matricardi, P.; Marianecci, C.; Alhaique, F. (2007). WebIn this regard, we chose as crowding agents a number of polymers with hydrodynamic dimensions comparable to those of iRFP713 in different structural states: polyethylene glycol8,000(PEG-8000)withaneffectivehydrodynamicradiusof24.5 1.9(Devanand & Selser, 1991), Dextran-40 and Dextran-70 with hydrodynamic radius of 44.550 and 5864 , Clipboard, Search History, and several other advanced features are temporarily unavailable. Learn More. National Library of Medicine By studying the molecular weights of various solutions of polymer, Solomon-Ciuta arrived at the formula which allowed the calculation of the intrinsic viscosity of polymer solutions by a single viscosity determination. The Solomon-Ciuta single-point method is used for calculating dextran intrinsic viscosity and the error relative is calculated, and %RE respect to Huggins method is >4.18% for 2000kDa, and >2.06% for 8.8kDa (Table 2). Journal of Colloid Science, 31, 308-328. From Table 1, It is worth noting that the value of 2 is above 0.95, being an acceptable value for viscosimetric measurement. Carbohydrate Polymers, 39, 377-380. DOI: 10.12691/jpbpc-1-1-3, Received September 18, 2013; Revised October 23, 2013; Accepted November 22, 2013. Fractional thermolysis by bipolar radiofrequency facilitates cutaneous delivery of peptide and siRNA with minor loss of barrier function. Arch Dermatol Res. 2012;101(8):28619. The hydrodynamic radius R h, defined as the radius of a hard sphere that diffuses at the same rate as the solute, takes these effects into account. European Polymer Journal, 42, 1086-1092. The Mark-Houwink parameters is calculated molecular weights range from 8.8 to 200kDa. Quintanilla-Pineda M, Achou CG, Daz J, Gutirrez-Falcon A, Bravo M, Herrera-Muoz JI, Pea-Navarro N, Alvarado C, Ibaez FC, Marzo F. Foods. dextran hydrodynamic radius. 20K. * shows a significant difference between the M.W. The above data are not useful because it can not be compared with those obtained in this work. For Research Use Only. After 24h, the cumulative permeation of FITC-dextran M.W. Journal of Polymer Science, 17, 527-546. A long-chain polymer molecule in solution takes on a somewhat kinked or curled shape, intermediate between a tightly curled mass (coil) and a rigid linear configuration. 2013;43(4):287303. 2003;10:33847. The calculation of Mark-Houwink parameters is carried out by the graphic representation of the following equation: where kMH (cm3/g) and a are Mark-Houwink constants, depending on the type of polymer, solvent, and temperature of intrinsic viscosity determinations [46]. Doke SK, Dhawale SC. Pharmaceutical Quality Dextran 40 is available from Pharmacosmos as active pharmaceutical ingredients (API) as excipient for final formulation, drug product, for medical devices, for production of solutions for injection and infusion and much more. FITC-dextran solution (600L) was added to the donor chamber. Carbohydrate Polymers, 74, 372-378. Solutions of dextrans and blue dextrans (0.21.0 g/L) were prepared in 0.200 M NaCl (aq). Viscosity of aqueous solutions of polysaccharides and hydrophobically modified polysaccharides: Application of Fedors equation. Springer Nature. Keratinocytes adjacent to the wound site induced by the microporation in the epidermis undergo a series of modifications that allow their proliferation and migration to the wound. Bahary, W. S.; Hogan, M. P.; Jilani, M.; Aronson, M. P. (1995). Results and Discussion The microscopic dynamics of proteins are probed in a light scattering experiment. Epub 2012 Oct 18. (1999). 4K and M.W. Table 2 compares the properties of nanoparticles coated with one or other polymer. Regulating dynamic signaling between hematopoietic stem cells All branched polysaccharide types could be described approximately by the hyperbranching theory. Sung-Wook Choi. Biophysical Journal, 87, 4259-4270. Ann Surg Oncol. Determination of the intrinsic viscosity has been the most used measurement in the last 60 years. (2013). Kasaai, Mohammad R. (2007). Takara, Andres; Acosta, Adolfo; Masuelli, Martin A. Polysaccharides-based nanoparticles as drug delivery systems. Table 2 compares the properties of nanoparticles coated with one or other polymer. Dextran Triple Detector Application Note 1, Viscotek GmbH, USA. The mechanism of the dextran-induced red blood cell Aggregation. The value of Mv is different from Mn, Mw, and Mz in a polydisperse polymer sample [38, 60, 61, 62]. The hydrodynamic radius (Rh) of individual samples of dextran, polyvinylpyrrolidone, and polyoxyethylene over a range of molecular weights (1,500 The Principle of the Suspended Level: Applications to the Measurement of Viscosity and Other Properties of Liquids. Kloster, C.; Bica C.; Rochas C.; Samios, D.; Geissler, E. (2000). Carbohydrate Polymers, 36, 319-326. There is a formula that used to calculate the radius (in Angstrom) from the molar mass (in g/mol) of dextran, a=0.33*(MM^0.46) where, a and MM are 2015 Mar 17;6:155. doi: 10.3389/fmicb.2015.00155. (2003). Multiple Linear Least-Squares Fits with a Common Intercept: Determination of the Intrinsic Viscosity of Macromolecules in Solution. The term f/f0 is sometimes denoted as P, Perrin constant. (2003). The importance of this type of study lies in the analysis of the polysaccharide behavior at industrial processes, the requirements to reduce energy, avoid flow problems and product quality control. 2016;9706:97061E6. These studies were again focused on investigating the hydrodynamic properties of the polymer molecules, their hydrodynamic radius in relation to their radius of gyration and the coherence of the simulation results with polymer theory. Analytes and stationary phase with Universal Calibration Relative Measurement Concentration and MW detector: RI + Viscometer Relationship between hydrodynamic volume (MW*intrinsic viscosity) and elution volume Multiple detection Dextran produced from Weissella cibaria JAG8 was purified and characterized. Pamis, R.; Hernndez Cifre, J. G.; Lpez Martnez, M.C. Journal of Chemical Education, 49(8), 577-579. Ghimici, Luminita; Nichifor, Marieta; Wolf, Bernhard (2009). These structural differences can significantly affect FITC-dextran delivery due to even after microporation [30]. Amersham Biosciences, AA Edition, www.amershambiosciences.com. Figure 3 shows that the parameters vary with temperature, evidencing a clear functionality of them. Carbohydrate Polymers 99, 199-207. Microporation techniques for enhanced delivery of therapeutic agents. Named after George Gabriel Stokes, it is closely related to solute mobility, factoring in not only size but also solvent effects.A smaller ion with stronger hydration, for example, may have a greater Stokes radius than a larger ion with Characterisation of insulin analogues therapeutically available to Weissella: An Emerging Bacterium with Promising Health Benefits. The particular dextrans which were used initially for conversion into synthetic blood-volume expanders [6, 7], human red blood cells aggregation for increasing the degree of polymerization and hence the molecular weight [8, 9, 10], hydrogels and microspheres [11], in drug transport system and its modifications as nanoparticles [12], in the removal through absorption of contaminant effluents such as heavy metals, organic molecules and inks [13], in the formation of biodegradable films [14, 15]. Masuelli, M. A. Levin G, Gershonowitz A, Sacks H, Stern M, Sherman A, Rudaev S, Zivin I, Phillip M. Transdermal delivery of human growth hormone through RF-microchannels. For a rigid or rod like polymer molecule that is greatly extended in solution, the Mark-Houwink a constant approaches to a value of 2.0 [48].The hydrodynamic radius (RH), for a sphere ((a/b) = 2.5) is given by the Einstein relation [49]. Masuelli, Martin Alberto. dextran Furthermore, the influence of temperature on the intrinsic viscosity is given by the chain flexibility parameter (dln[]/dT), which gives information about the conformation of the macromolecule chain in solution [62]. dextran hydrodynamic radius Kloster, C.; Bica, C.; Lartigue, C.; Rochas, C.; Samios, D.; Geissler E. (1998). In this work, we evaluate the maximum radius of macromolecules able to enter into HeLa cells following a plasma treatment using a size exclusion approach with dextran molecules. Advances in Cosmetic Surgery. II. FITC-derivatives have been used for a variety of biological applications for more than 40 yeras. Appl Biochem Biotechnol. Determination of intrinsic viscosities of macromolecules and nanoparticles. ; Wood, P.J. One day before the experiment, the skin was stabilized in the skin culture medium. Polysaccharide hydrogels for modified release formulations. Middelkoop E, Bogaerdt AJ, Lamme EN, Hoekstra MJ, Brandsma K, Ulrich MMW. The M-H equation is empirical and valid for monodisperse polymers, and it is applied to biomacromolecules (complex, hyperbranched, crosslink), taking the precaution of comparing the value of Mv with the polydispersity index (qM-H). In contrast to linear chains, power law behavior is observed only asymptotically at large Mv. The intrinsic viscosity calculation requires several concentrations, and because of this inconvenient will not use the Huggins method in this paper. Biomaterials. Alternatively, the value of qM-H can be calculated using numerical methods and other-average molecular weights (Mn, Mw, Mz) according to where c and d are empirical polynomial functions of the exponent a. Hydrodynamic Radius Epub 2022 Feb 2. The human and pig skin samples were placed in the receiver chamber of the Transdermal diffusion cell system with the SC facing up and the donor chamber was fixed in place. 2012;9(2):14754. American Journal of Food Science and Technology 2013, 1(3), 60-66. WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. Journal of Polymer Science Part B: Polymer Physics, 38, 2591-2598. Manage cookies/Do not sell my data we use in the preference centre. On the molecular weight distribution of dextran T-500. Webof overall macromolecular geometry and hydrodynamic draining. Kumar SV, Tarun P, Kumar TA. We believe that the microporation device can be used as a potential technique for effective transdermal drug delivery. Kasaai, M. R.; Arul, J.; Charlet, G. (2000). Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. Food Sci Biotechnol. It is a measure of the width of molecular weight distributions (MWD) as well as probability of molecular weight distribution curve (GPC). Biotechnology Progress, 19, 544-548. Alternatives to animal testing: a review. Figure3 shows a magnified image of human cadaver skin with microchannels formed by the microporation device (250kHz) and the skin closure over time. Mark-Houwink-Kuhn-Sakurada Exponent at the Condition. Dear Sir. Alan F Rawle , Can you help me in calculating the size of dextran 4 (molecular weight= 4000) and dextran 10 (molecular weight 10,000)? Th The relative browning for dextran from W. cibaria JAG8 was similar to commercial prebiotic Nutraflora P-95 and 3-fold lower than Raftilose P-95. Fusco V, Quero GM, Cho GS, Kabisch J, Meske D, Neve H, Bockelmann W, Franz CM. The temperature increment can induce a macromolecule compaction (decreases in RH and []), generating by this way, a greater difficult to flowing due at requires an increase of energy consumption (Eavf<). The Mark-Houwink. CAS Journal of Physical Chemistry. Im Tiefkhlgert (-5 bis -30C) lagern und vor Licht schtzen. Before Thermo Fisher Scientific. 2004;25(9):155967. (2013). An undergraduate physical chemistry experiment. (2013). Deng H, Konopka CJ, Prabhu S, Sarkar S, Medina NG, Fayyaz M, Arogundade OH, Vidana Gamage HE, Shahoei SH, Nall D, Youn Y, Dobrucka IT, Audu CO, Joshi A, Melvin WJ, Gallagher KA, Selvin PR, Nelson ER, Dobrucki LW, Swanson KS, Smith AM. Polymers | Free Full-Text | Confinement-Induced Fractionation and Hydrodynamic cavitation (HC), a common phenomenon that can produce local excessive temperature, high pressure and large amount of energy. Fluorescent dextran (2006). TdB Labs produce a wide range of FITC-derivatives (490 nm/520 nm) with a mean molecular weight ranging from 4 kDa to 2000 kDa. WebHydrodynamic radius = variable size of an analyte with specific MW based on dynamical properties. Barrier integrity was examined using Tewameter to confirm the poration of human cadaver skin using a microporation device. Lim SB, Tingirikari JMR, Seo JS, Li L, Shim S, Seo JH, Han NS. Increased epidermal laser fluence through simultaneous ultrasonic microporation. Analytical Chemistry. Careers. Figure5 shows the cumulative amount of the FITC-dextran (M.W. Pig skins (Micropig Franz cell membrane) were supplied by Medi Kinetics Co., Ltd. (Pyeongtaek, Korea). Porcine wound models for skin substitution and burn treatment. Modelling the endothelial blood-CNS barriers: a method for the Changes in the Mark-Houwink hydrodynamic volume of chitosan molecules in solutions of different organic acids, at different temperatures and ionic strengths. Part of FITC-dextran solution was prepared at 1mM in PBS. After microporation, the TEWL values of the human cadaver skin increased from 11.30.6 to 33.01.0, 10.71.5 to 45.31.5, and 10.70.6 to 61.02.0g/m2/h when the microporation was performed 2, 5, and 10 times, respectively. Dextran
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